Cell Type

Media

Additives

C2C12 (Growth Media)

DMEM

4500g/L Glucose

10% FBS

1% Pen/Strep

C2C12 (Differentiation Media)

DMEM

4500g/L Glucose

2% Horse Serum

1% Pen/Strep

3T3 Fibroblasts

DMEM

4500g/L Glucose

10% FBS

1% Pen/Strep

Primary muscle (Growth Media)

DMEM:F12

20% FBS

2% Ultroser G

1% Pen/Strep

Primary muscle (Differentiation Media)

Media 199

10% FBS

1% Pen/Strep

Primary tendon (growth media)

F12 (Hams)

20% FBS

1% ABAM

Primary tendon (differentiation media)

F12K

10% Heat inactive FBS

1% ABAM

50g/ml Proline

50g/ml Vitamin C